Inducible kidney-specific Sgk1 knockout mice show a salt-losing phenotype Nourdine Faresse,1 Dagmara Lagnaz,1 Anne Debonneville,1 Adil Ismailji,1 Marc Maillard,2 Geza Fejes-Toth,3 Aniko Náray-Fejes-Tóth,3 and Olivier Staub1 1Department of Pharmacology and Toxicology, University of Lausanne and 2Nephrology Department, CHUV, Lausanne, Switzerland; and 3Department of … The LoxP mouse line has two loxP elements flanking the entire or part of the gene of interest, giving the loxP mouse defined gene(s) to be edited. The inducible analog of Cre is ERT2-Cre-ERT2, in which Cre activity will only be observed with the introduction of Tamoxifen. Christian Günschmann. Salt‐inducible kinase 1 knockout mice show a salt‐retaining phenotype and salt‐sensitive hypertension (1136.5) Maria Pinho. Besides the generation of the inducible knockout mice of interest (Section 3.1.2), it is highly recommended to validate the recombinationproperties of the Cre mouse, that is, the sites of recombinase expression and tamoxifen-dependent Cre activity, by crossing it … By crossing PTEN conditional knockout mice with transgenic mice expressing a tamoxifen-inducible Cre-ER(T) under the control of a chicken actin promoter, we have generated a tamoxifen-inducible mouse model that allows temporal control of PTEN deletion. Inducible Knockout of Mouse Zfhx3 Emphasizes Its Key Role in Setting the Pace and Amplitude of the Adult Circadian Clock Ashleigh G. Wilcox, Lucie Vizor, Michael J. Parsons,1 Gareth Banks, and Patrick M. Nolan2 MRC Harwell Institute, Harwell Science Campus, Oxfordshire, UK Abstract The transcription factor zinc finger homeobox 3 (ZFHX3) plays a key Correspondence. Transgenic Mouse Technology in Skin Biology: Inducible Gene Knockout in Mice. Author information: (1)1 Vertex Pharmaceuticals, Boston, MA 02210. Besides the generation of the inducible knockout mice of interest (Section 3.1.2), it is highly recommended to validate the recombination properties of the Cre mouse, that is, the sites of recombinase expression and tamoxifen-dependent Cre activity, by crossing it … BIAL, Department of Research and Development, Porto, Portugal. Search for more papers by this author. Conditional/inducible mutation approaches are then required that first allow the mouse to develop and mature normally prior to ablation of the gene of interest. By crossing PTEN conditional knockout mice with transgenic mice expressing a tamoxifen-inducible Cre-ER T under the control of a chicken actin promoter, we have generated a tamoxifen-inducible mouse model that allows temporal control of PTEN deletion. Department of Pharmacology and Therapeutics, Porto, Portugal. With the Tet-OFF system, you can administer doxycycline to the mice from birth until the time you want Cre activity to knockout your target gene. Inducible Knockout of Mouse Zfhx3 Emphasizes Its Key Role in Setting the Pace and Amplitude of the Adult Circadian Clock. We designed multiple drug-inducible sgRNA expression vectors and measured their activities using an EGFP gene disruption assay in 11 human and mouse cell lines. Department of Dermatology, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Joseph-Stelzmann-Strasse 26, 50931 Cologne, Germany. Wilcox AG, Vizor L, Parsons MJ(1), Banks G, Nolan PM. Another serious limitation is a lack of evolutive adaptations in knockout model that might occur in wild type animals after they naturally mutate. mouse’’). The optimal design allows for a tight and inducible control of gene knockout in vitro, and in vivo during a seven-week-long experiment following hematopoietic reconstitution in mice. 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